An analysis of a record-breaking gravitational-wave detection tests whether general relativity holds under extreme conditions.
Category: futurism
A ribosome-bound pseudoknot in the HCV coding region stimulates viral growth by tuning viral translation
Given that pk1 is located within the HCV coding region, we next investigated a potential role in translation elongation. We inserted pk1 into the ORF of Gaussia luciferase and used the HCV IRES to initiate translation (Figure 3 B, left). To ensure that the highly structured HCV IRES did not affect pk1 folding, we included a 99-nt linker sequence with low structural propensity between the HCV IRES and pk1. As controls, we used the pk1-unzip mutant and a randomized pk1, where the pk1 sequence was shuffled by three nucleotides, thereby maintaining the same amino acid composition as the WT pk1.
The RNA constructs described above were transfected into cells, and luciferase activity was measured at various time points to assess translation activity (Figure 3B, right). We observed that pk1 significantly inhibited translation elongation at all time points tested compared to the pk1-unzip and random controls. Notably, at 4 h post-transfection, there was nearly a 4-fold difference between the activity of pk1 and the pk1-unzip mutant, indicating that the pseudoknot structure itself, rather than just its constituent stems, is crucial for the observed translation inhibition (Figure 3B). To further examine the structural basis of this effect, we introduced a pk1-compensatory mutant that restores the disrupted base pairing in the pk1-unzip mutant using synonymous G-U wobble and A-U base pairs (sequence shown in Figure 3A).
Why Do Galactic Empires Collapse? | Explaining The Fall of Space Empires
👽 Please consider supporting this channel on Patreon: https://www.patreon.com/ideasoficeandfire.
or PAYPAL — https://paypal.me/QuinnsIdeas?locale.x=en_US
🎨 Art:
🎵 Music: https://www.youtube.com/@JamezDahlMusic.
📚 Get These Books! Affiliate link
https://amzn.to/3AQ0aSs.
Quinn’s Discord: https://discord.gg/uGx4WsjVCZ
FOLLOW QUINN ON TWITTER: Twitter: https://twitter.com/IDEASOFICE_FIRE
I NOW HAVE A SUBREDDIT: https://www.reddit.com/r/IdeasofIceandFire/
Quinn’s New Graphic Novel: https://www.quinnhoward.net/theliebehindthestar.
Buy Quinn’s Comic Books: https://www.quinnhoward.net/shop.
Quinn’s Website: https://www.quinnhoward.net.
Like me on Facebook!: https://www.facebook.com/IOIAF
🎥 Mentioned Videos.
🎬 Other Playlist.
CERN chief upbeat on funding for new particle collider
Mark Thomson, the new head of Europe’s physics laboratory CERN, voiced confidence Tuesday about raising the billions of dollars needed to build by far the world’s biggest particle accelerator.
CERN, the European Organization for Nuclear Research, seeks to unravel what the universe is made of and how it works.
The planned Future Circular Collider (FCC) would be an electron-positron collider ring with a circumference of 91 kilometers and an average depth of 200 meters.