Lifeboat Foundation

An attempt was made to reprogram peripheral blood cells into human induced pluripotent stem cell (hiPSCs) as a new cell source for cartilage repair.

We generated chondrogenic lineage from human peripheral blood via hiPSCs using an integration-free method. Peripheral blood cells were either obtained from a human blood bank or freshly collected from volunteers. After transforming peripheral blood cells into iPSCs, the newly derived iPSCs were further characterized through karyotype analysis, pluripotency gene expression and cell differentiation ability. iPSCs were differentiated through multiple steps, including embryoid body formation, hiPSC-mesenchymal stem cell (MSC)-like cell expansion, and chondrogenic induction for 21 days. Chondrocyte phenotype was then assessed by morphological, histological and biochemical analysis, as well as the chondrogenic expression.

HiPSCs derived from peripheral blood cells were successfully generated, and were characterized by fluorescent immunostaining of pluripotent markers and teratoma formation in vivo. Flow cytometric analysis showed that MSC markers CD73 and CD105 were present in monolayer cultured hiPSC–MSC-like cells. Both alcian blue and toluidine blue staining of hiPSC–MSC-chondrogenic pellets showed as positive. Immunohistochemistry of collagen II and X staining of the pellets were also positive. The sulfated glycosaminoglycan content was significantly increased, and the expression levels of the chondrogenic markers COL2, COL10, COL9 and AGGRECAN were significantly higher in chondrogenic pellets than in undifferentiated cells. These results indicated that peripheral blood cells could be a potential source for differentiation into chondrogenic lineage in vitro via generation of mesenchymal progenitor cells.

Most 3D printing methods currently in use rely either on photo (light)- or thermo (heat)-activated reactions to achieve precise manipulation of polymers. The development of a new platform technology called direct sound printing (DSP), which uses soundwaves to produce new objects, may offer a third option.

The process is described in a paper published in Nature Communications. It shows how focused ultrasound waves can be used to create sonochemical reactions in minuscule cavitation regions—essentially tiny bubbles. Extremes of temperature and pressure lasting trillionths of a second can generate pre-designed complex geometries that cannot be made with existing techniques.

“Ultrasonic frequencies are already being used in destructive procedures like laser ablation of tissues and tumors. We wanted to use them to create something,” says Muthukumaran Packirisamy, a professor and Concordia Research Chair in the Department of Mechanical, Industrial and Aerospace Engineering at the Gina Cody School of Engineering and Computer Science. He is the paper’s corresponding author.

Plastic bottles, punnets, wrap – such lightweight packaging made of PET plastic becomes a problem if it is not recycled. Scientists at Leipzig University have now discovered a highly efficient enzyme that degrades PET in record time. The enzyme PHL7, which the researchers found in a compost heap in Leipzig, could make biological PET recycling possible much faster than previously thought. The findings have now been published in the scientific journal “ChemSusChem” and selected as the cover topic.

One way in which enzymes are used in nature is by bacteria to decompose plant parts. It has been known for some time that some enzymes, so-called polyester-cleaving hydrolases, can also degrade PET. For example, the enzyme LCC, which was discovered in Japan in 2012, is considered to be a particularly effective “plastic eater”. The team led by Dr Christian Sonnendecker, an early career researcher from Leipzig University, is searching for previously undiscovered examples of these biological helpers as part of the EU-funded projects MIPLACE and ENZYCLE. They found what they were looking for in the Südfriedhof, a cemetery in Leipzig: in a sample from a compost heap, the researchers came across the blueprint of an enzyme that decomposed PET at record speed in the laboratory.

The researchers from the Institute of Analytical Chemistry found and studied seven different enzymes. The seventh candidate, called PHL7, achieved results in the lab that were significantly above average. In the experiments, the researchers added PET to containers with an aqueous solution containing either PHL7 or LCC, the previous leader in PET decomposition. Then they measured the amount of plastic that was degraded in a given period of time and compared the values with each other.

Chemical reactions that are driven by light offer a powerful tool for chemists who are designing new ways to manufacture pharmaceuticals and other useful compounds. Harnessing this light energy requires photoredox catalysts, which can absorb light and transfer the energy to a chemical reaction.

MIT chemists have now designed a new type of photoredox catalyst that could make it easier to incorporate light-driven reactions into manufacturing processes. Unlike most existing photoredox catalysts, the new class of materials is insoluble, so it can be used over and over again. Such catalysts could be used to coat tubing and perform chemical transformations on reactants as they flow through the tube.

“Being able to recycle the catalyst is one of the biggest challenges to overcome in terms of being able to use photoredox catalysis in manufacturing. We hope that by being able to do flow chemistry with an immobilized catalyst, we can provide a new way to do photoredox catalysis on larger scales,” says Richard Liu, an MIT postdoc and the joint lead author of the new study.

Every lungful of air we suck down is mostly made up of nitrogen, with a generous helping of oxygen, and a dash of carbon dioxide.

But dusting this atmospheric soup is a whole encyclopedia of different compounds and elements, some of which we can only speculate about.

One of those mysteries just came into focus, however. Chemists have shown that a reactive class of compounds called organic hydrotrioxides exists in the atmosphere, and while these chemicals last only briefly, they could have effects we don’t know about.

A completely new kind of molecule has been made by combining an extremely cold ion and a super-sized atom. The unusual molecular bond between the two particles was thousands of times longer than those in most room-temperature molecules, and the method to make and study it could kick-start a new branch of ultracold quantum chemistry.

Reprogramming without having to insert genes.

When people think of cellular reprogramming, converting a differentiated cell into a stem cell, they often refer to the overexpression of Yamanaka factors[Oct4, Klf4, Sox2 & c-Myc]. Rightly so. But what if i told you that stem cells could be induced with just chemicals. Well you would reply “show me the data”. So, let’s take a look at this recent Nature paper that showed how combinations of small molecules/chemicals converted human differentiated cells to stem cells.

Continue reading “Making stem cells with chemicals” »

Oscillations are widespread throughout the natural world and a number of fascinating inorganic oscillating reactions are known—but the formation and control of oscillating, self-replicating synthetic systems has remained challenging. Now, it has been shown that chemically fuelled oscillations within a network of organic replicators can drive supramolecular assembly and disassembly.

What do you do when a tried-and-true method for determining the sun’s chemical composition appears to be at odds with an innovative, precise technique for mapping the sun’s inner structure? That was the situation facing astronomers studying the sun—until new calculations that have now been published by Ekaterina Magg, Maria Bergemann and colleagues, and that resolve the apparent contradiction.

The decade-long solar abundance crisis is the conflict between the internal structure of the sun as determined from solar oscillations (helioseismology) and the structure derived from the fundamental theory of stellar evolution, which in turn relies on measurements of the present-day sun’s chemical composition. The new calculations of the physics of the sun’s atmosphere yield updated results for abundances of different chemical elements, which resolve the conflict. Notably, the sun contains more oxygen, silicon and neon than previously thought. The methods employed also promise considerably more accurate estimates of the chemical compositions of stars in general.